Step 1: Camera Installation

Required Equipment

• Basler Camera (or other compatible camera)
• 0.5x C-mount adapter (recommended)
• Camera compatible cable (USB 3.0)

Installation

1. Attach the C-mount adapter to the microscope’s camera port
2. Connect the camera to the adapter
3. Connect the USB 3.0 cable to the camera and your computer (blue port)
4. Check illumination – set to maximum, direct 100% light to the camera

Connection Test

• The camera should be detected by the system
• Not working? Check all connections and ensure you’re using a USB 3.0 port

Step 2: PathoCam Installation

Windows

1. Download drivers: Basler Pylon Runtime
2. Download PathoCam
3. Run the installer → ignore security warnings (click “More info” → “Run anyway”)

macOS

1. Download PathoCam
2. Install → if the application is blocked: System Preferences → Privacy & Security → “Open Anyway”

Step 3: First Launch

1. Connect the camera to a USB 3.0 port (blue-colored port)
2. Launch PathoCam → click “Log In”
3. You will be redirected to the CancerCenter.ai platform
4. Select your organization and target folder for scans

Step 4: First Scan

1. Place the slide under the microscope
2. Check image orientation in PathoCam – if the image is rotated:
   • DO NOT adjust by tightening the camera
   • Use an Allen key to adjust the C-mount adapter
   • Rotate the adapter until the image is correctly oriented
3. Set illumination and focus
4. Adjust image settings in the application
5. In PathoCam, click “Start”
6. Gently move the microscope stage – the application automatically stitches the image
7. When finished, click “End”, name the scan, and press “Save”

✅ Done! Your scan is available on the CancerCenter.ai platform